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rabbit anti rab11  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti rab11
    Rabbit Anti Rab11, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rab11/Rab7+Mouse+mAb/bio_rxiv__64898__2026__03__22__713512-167-63-66
    Average 95 stars, based on 75 article reviews
    rabbit anti rab11 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model.
    Article Snippet: PLOS Biology | https://doi.org/10.1371/journal.pbio.3003603 January 27, 2026 15 / 25 The following antibodies were used for flow cytometry analyses: APC anti-human CD274 (329708, 1:100, Biolegend, RRID: AB_940360) and APC mouse IgG2b (400322, 1:100, Biolegend, RRID: AB_326500). .. The following antibodies were used for immunofluorescence staining: anti-PD-L1 (LS-C338364, 1:100, LSBio, RRID: AB_2941014), anti-LAMP1 (9091S, 1:100, CST, RRID: AB_2687579), anti-RAB11 (5589S, 1:50, CST), anti-RAB7B (ab137029, 1:100, Abcam, RRID: AB_2629474), anti-HSD17B12 (ab236990, 1:100, Abcam, RRID: AB_2876866), anti-CMTM6 (HPA026980, 1:100, Sigma, RRID: AB_10602801), anti-CD3 (ab237721, 1:4000, Abcam), anti-CD8 (ZA0508, ZSGB-BIO, RRID: AB_2890107), anti-GZMB (ab255598, 1:8000, Abcam, RRID: AB_2860567), IgG H&L (HRP) (ab205718, 1:4000, Abcam, RRID: AB_2819160), goat anti-mouse highly cross-adsorbed Alexa Fluor Plus 488 (A32723, 1:500, Thermofisher, RRID: AB_2633275), goat anti-rabbit IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 594 (A32740, 1:500, Thermofisher, RRID: AB_2762824), goat anti-rabbit IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 647 (A32728, 1:500, Thermofisher, RRID: AB_2633277), goat anti-human IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 488 (A11013, 1:500, Thermofisher, RRID: AB_141360). .. The following antibodies were used for immunohistochemistry staining: anti-HSD17B12 (ab236990, 1:100, Abcam, RRID: AB_2876866).

    Article Title: The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model
    Article Snippet: The following antibodies were used for flow cytometry analyses: APC anti-human CD274 (329708, 1:100, Biolegend, RRID: AB_940360) and APC mouse IgG2b (400322, 1:100, Biolegend, RRID: AB_326500). .. The following antibodies were used for immunofluorescence staining: anti-PD-L1 (LS-C338364, 1:100, LSBio, RRID: AB_2941014), anti-LAMP1 (9091S, 1:100, CST, RRID: AB_2687579), anti-RAB11 (5589S, 1:50, CST), anti-RAB7B (ab137029, 1:100, Abcam, RRID: AB_2629474), anti-HSD17B12 (ab236990, 1:100, Abcam, RRID: AB_2876866), anti-CMTM6 (HPA026980, 1:100, Sigma, RRID: AB_10602801), anti-CD3 (ab237721, 1:4000, Abcam), anti-CD8 (ZA-0508, ZSGB-BIO, RRID: AB_2890107), anti-GZMB (ab255598, 1:8000, Abcam, RRID: AB_2860567), IgG H&L (HRP) (ab205718, 1:4000, Abcam, RRID: AB_2819160), goat anti-mouse highly cross-adsorbed Alexa Fluor Plus 488 (A32723, 1:500, Thermofisher, RRID: AB_2633275), goat anti-rabbit IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 594 (A32740, 1:500, Thermofisher, RRID: AB_2762824), goat anti-rabbit IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 647 (A32728, 1:500, Thermofisher, RRID: AB_2633277), goat anti-human IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 488 (A11013, 1:500, Thermofisher, RRID: AB_141360). .. The following antibodies were used for immunohistochemistry staining: anti-HSD17B12 (ab236990, 1:100, Abcam, RRID: AB_2876866).

    Staining:

    Article Title: The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model.
    Article Snippet: PLOS Biology | https://doi.org/10.1371/journal.pbio.3003603 January 27, 2026 15 / 25 The following antibodies were used for flow cytometry analyses: APC anti-human CD274 (329708, 1:100, Biolegend, RRID: AB_940360) and APC mouse IgG2b (400322, 1:100, Biolegend, RRID: AB_326500). .. The following antibodies were used for immunofluorescence staining: anti-PD-L1 (LS-C338364, 1:100, LSBio, RRID: AB_2941014), anti-LAMP1 (9091S, 1:100, CST, RRID: AB_2687579), anti-RAB11 (5589S, 1:50, CST), anti-RAB7B (ab137029, 1:100, Abcam, RRID: AB_2629474), anti-HSD17B12 (ab236990, 1:100, Abcam, RRID: AB_2876866), anti-CMTM6 (HPA026980, 1:100, Sigma, RRID: AB_10602801), anti-CD3 (ab237721, 1:4000, Abcam), anti-CD8 (ZA0508, ZSGB-BIO, RRID: AB_2890107), anti-GZMB (ab255598, 1:8000, Abcam, RRID: AB_2860567), IgG H&L (HRP) (ab205718, 1:4000, Abcam, RRID: AB_2819160), goat anti-mouse highly cross-adsorbed Alexa Fluor Plus 488 (A32723, 1:500, Thermofisher, RRID: AB_2633275), goat anti-rabbit IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 594 (A32740, 1:500, Thermofisher, RRID: AB_2762824), goat anti-rabbit IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 647 (A32728, 1:500, Thermofisher, RRID: AB_2633277), goat anti-human IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 488 (A11013, 1:500, Thermofisher, RRID: AB_141360). .. The following antibodies were used for immunohistochemistry staining: anti-HSD17B12 (ab236990, 1:100, Abcam, RRID: AB_2876866).

    Article Title: The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model
    Article Snippet: The following antibodies were used for flow cytometry analyses: APC anti-human CD274 (329708, 1:100, Biolegend, RRID: AB_940360) and APC mouse IgG2b (400322, 1:100, Biolegend, RRID: AB_326500). .. The following antibodies were used for immunofluorescence staining: anti-PD-L1 (LS-C338364, 1:100, LSBio, RRID: AB_2941014), anti-LAMP1 (9091S, 1:100, CST, RRID: AB_2687579), anti-RAB11 (5589S, 1:50, CST), anti-RAB7B (ab137029, 1:100, Abcam, RRID: AB_2629474), anti-HSD17B12 (ab236990, 1:100, Abcam, RRID: AB_2876866), anti-CMTM6 (HPA026980, 1:100, Sigma, RRID: AB_10602801), anti-CD3 (ab237721, 1:4000, Abcam), anti-CD8 (ZA-0508, ZSGB-BIO, RRID: AB_2890107), anti-GZMB (ab255598, 1:8000, Abcam, RRID: AB_2860567), IgG H&L (HRP) (ab205718, 1:4000, Abcam, RRID: AB_2819160), goat anti-mouse highly cross-adsorbed Alexa Fluor Plus 488 (A32723, 1:500, Thermofisher, RRID: AB_2633275), goat anti-rabbit IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 594 (A32740, 1:500, Thermofisher, RRID: AB_2762824), goat anti-rabbit IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 647 (A32728, 1:500, Thermofisher, RRID: AB_2633277), goat anti-human IgG (H + L) highly cross-adsorbed Alexa Fluor Plus 488 (A11013, 1:500, Thermofisher, RRID: AB_141360). .. The following antibodies were used for immunohistochemistry staining: anti-HSD17B12 (ab236990, 1:100, Abcam, RRID: AB_2876866).

    Control:

    Article Title: Synaptic Vesicle Glycoprotein 2A Suppresses Amyloidogenesis Beyond Its Synaptic Role: A Novel Mechanism Disrupting BACE1 Binding and Altering APP Localization
    Article Snippet: .. The following antibodies were used for this experiment: anti‐SV2A (Cat. No. ab32942, Abcam, Cambridge, MA, USA, 1:3000); anti‐APP (Cat. No. ab32136, Abcam, 1:5000); IgG isotype control (Cat. No. 10400C, Invitrogen, Carlsbad, CA, USA, 1:5000); anti‐β‐actin (Cat. No. TA‐09, ZSGB‐BIO, Shanghai, China, 1:8000), anti‐GAPDH (Cat. No. 60004‐1‐Ig, Proteintech, Wuhan, China, 1:8000), anti‐BACE1 (Cat. No. ab183612, Abcam, 1:800); anti‐EEA1 (Cat. No. 610456, BD Biosciences, 1:1000); anti‐Rab7 (ab137029, Abcam, 1:2000); anti‐Rab11 (Cat. No. 5589, Cell Signaling Technology, 1:1000); anti‐LAMP1 (Cat. No. sc2011, Santa Cruz, 1:1000); myc tag polyclonal antibody (Cat. No. 16286‐1‐AP, Proteintech, Wuhan, China, 1:3000); DYKDDDDK tag polyclonal antibody (20543‐1‐AP, Proteintech, Wuhan, China, 1:3000). ..

    Incubation:

    Article Title: Activated microglial exosomes enhance α-Synuclein internalization and accelerate neurodegeneration in lewy body dementia
    Article Snippet: The appropriate volume of viral supernatant was added to the culture plates (MOI = 50), and the cells were incubated at 37 °C with 5% CO2 for 8–16 h. Subsequently, 1.5 μg/ml puromycin (Thermo Fisher) was added to each well for selection of stable cell lines. .. Briefly, cells were fixed with 4% paraformaldehyde (PFA) for 15 min, permeabilized with 0.5% Triton X-100 for 20 min, and blocked with 3% BSA for 1 h. The dishes were incubated overnight at 4 °C with the following primary antibodies: anti-LAG3 (1:100, Abcam, ab209238), anti-p-a-syn (1:1000, Abcam, ab51253), anti-Map-2 (1:2000, Abcam, ab5392), anti-ChAT (1:800, ThermoFisher, MA5-31382), anti-Iba1 (1:1000, Abcam, ab107159), anti-EEA-1 (1:500, Abcam, ab109110), anti-RAB7 (1:200, Abcam, ab137029), anti-LAMP-1 (1:100, Abcam, ab208943), and anti-RAB11 (1:50, Cell Signaling Technology, 5589 S). .. Briefly, cells were fixed with 4% paraformaldehyde (PFA) for 15 min, permeabilized with 0.5% Triton X-100 for 20 min, and blocked with 3% BSA for 1 h. The dishes were incubated overnight at 4 °C with the following primary antibodies: anti-LAG3 (1:100, Abcam, ab209238), anti-p-a-syn (1:1000, Abcam, ab51253), anti-Map-2 (1:2000, Abcam, ab5392), anti-ChAT (1:800, ThermoFisher, MA5-31382), anti-Iba1 (1:1000, Abcam, ab107159), anti-EEA-1 (1:500, Abcam, ab109110), anti-RAB7 (1:200, Abcam, ab137029), anti-LAMP-1 (1:100, Abcam, ab208943), and anti-RAB11 (1:50, Cell Signaling Technology, 5589 S).

    Article Title: Activated microglial exosomes enhance α-Synuclein internalization and accelerate neurodegeneration in lewy body dementia.
    Article Snippet: The appropriate volume of viral supernatant was added to the culture plates (MOI = 50), and the cells were incubated at 37 °C with 5% CO2 for 8–16 h. Subsequently, 1.5 μg/ml puromycin (Thermo Fisher) was added to each well for selection of stable cell lines. .. Briefly, cells were fixed with 4% paraformaldehyde (PFA) for 15 min, permeabilized with 0.5% Triton X-100 for 20 min, and blocked with 3% BSA for 1 h. The dishes were incubated overnight at 4 °C with the following primary antibodies: anti-LAG3 (1:100, Abcam, ab209238), anti-p-a-syn (1:1000, Abcam, ab51253), antiMap-2 (1:2000, Abcam, ab5392), anti-ChAT (1:800, ThermoFisher, MA5-31382), anti-Iba1 (1:1000, Abcam, ab107159), anti-EEA-1 (1:500, Abcam, ab109110), antiRAB7 (1:200, Abcam, ab137029), anti-LAMP-1 (1:100, Abcam, ab208943), and anti-RAB11 (1:50, Cell Signaling Technology, 5589 S). .. Briefly, cells were fixed with 4% paraformaldehyde (PFA) for 15 min, permeabilized with 0.5% Triton X-100 for 20 min, and blocked with 3% BSA for 1 h. The dishes were incubated overnight at 4 °C with the following primary antibodies: anti-LAG3 (1:100, Abcam, ab209238), anti-p-a-syn (1:1000, Abcam, ab51253), antiMap-2 (1:2000, Abcam, ab5392), anti-ChAT (1:800, ThermoFisher, MA5-31382), anti-Iba1 (1:1000, Abcam, ab107159), anti-EEA-1 (1:500, Abcam, ab109110), antiRAB7 (1:200, Abcam, ab137029), anti-LAMP-1 (1:100, Abcam, ab208943), and anti-RAB11 (1:50, Cell Signaling Technology, 5589 S).



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    Effect of SV2A overexpression on the distribution of APP. (a, b) Expressions of EEA1, Rab7, Rab11, and LAMP1 in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and their control cells, as well as the relative expression analysis. (c, d) Fluorescence intensity of EEA1 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of EEA1 and APP. (e, f) Fluorescence intensities of Rab7 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of Rab7 and APP. (g, h) Fluorescence intensity <t>of</t> <t>Rab11</t> and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of Rab11 and APP. (i–j) Fluorescence intensities of LAMP1 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of LAMP1 and APP. (k) Quantification of surface APP expression in SH‐SY5Y cells transfected with SV2Aoe or control plasmids. (l) Quantification of surface APP fluorescence intensity (APP/DAPI) in SH‐SY5Y cells transfected with SV2Aoe or control plasmids, scale bar: 10 μm. (m) Fluorescence level of APP on the cell surface in hippocampal regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con; scale bar: 50 μm. (n) Relative fluorescence intensity of APP (APP/DAPI) on the cell surface in the hippocampal regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con. (o) Fluorescence level of APP on the cell surface in cortical regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con; scale bar: 50 μm. (p) Relative fluorescence intensity of APP (APP/DAPI) on the cell surface in the cortical regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con. The protein levels of APP on the cell surface were measured by the cell surface biotin assay. Data are presented as the mean ± SD. All dot plots: t ‐test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Image Search Results


    Effect of SV2A overexpression on the distribution of APP. (a, b) Expressions of EEA1, Rab7, Rab11, and LAMP1 in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and their control cells, as well as the relative expression analysis. (c, d) Fluorescence intensity of EEA1 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of EEA1 and APP. (e, f) Fluorescence intensities of Rab7 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of Rab7 and APP. (g, h) Fluorescence intensity of Rab11 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of Rab11 and APP. (i–j) Fluorescence intensities of LAMP1 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of LAMP1 and APP. (k) Quantification of surface APP expression in SH‐SY5Y cells transfected with SV2Aoe or control plasmids. (l) Quantification of surface APP fluorescence intensity (APP/DAPI) in SH‐SY5Y cells transfected with SV2Aoe or control plasmids, scale bar: 10 μm. (m) Fluorescence level of APP on the cell surface in hippocampal regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con; scale bar: 50 μm. (n) Relative fluorescence intensity of APP (APP/DAPI) on the cell surface in the hippocampal regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con. (o) Fluorescence level of APP on the cell surface in cortical regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con; scale bar: 50 μm. (p) Relative fluorescence intensity of APP (APP/DAPI) on the cell surface in the cortical regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con. The protein levels of APP on the cell surface were measured by the cell surface biotin assay. Data are presented as the mean ± SD. All dot plots: t ‐test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Aging Cell

    Article Title: Synaptic Vesicle Glycoprotein 2A Suppresses Amyloidogenesis Beyond Its Synaptic Role: A Novel Mechanism Disrupting BACE1 Binding and Altering APP Localization

    doi: 10.1111/acel.70379

    Figure Lengend Snippet: Effect of SV2A overexpression on the distribution of APP. (a, b) Expressions of EEA1, Rab7, Rab11, and LAMP1 in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and their control cells, as well as the relative expression analysis. (c, d) Fluorescence intensity of EEA1 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of EEA1 and APP. (e, f) Fluorescence intensities of Rab7 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of Rab7 and APP. (g, h) Fluorescence intensity of Rab11 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of Rab11 and APP. (i–j) Fluorescence intensities of LAMP1 and APP in SV2A‐overexpressed SH‐SY5Y cells (N2a cells) and the co‐localization analysis of LAMP1 and APP. (k) Quantification of surface APP expression in SH‐SY5Y cells transfected with SV2Aoe or control plasmids. (l) Quantification of surface APP fluorescence intensity (APP/DAPI) in SH‐SY5Y cells transfected with SV2Aoe or control plasmids, scale bar: 10 μm. (m) Fluorescence level of APP on the cell surface in hippocampal regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con; scale bar: 50 μm. (n) Relative fluorescence intensity of APP (APP/DAPI) on the cell surface in the hippocampal regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con. (o) Fluorescence level of APP on the cell surface in cortical regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con; scale bar: 50 μm. (p) Relative fluorescence intensity of APP (APP/DAPI) on the cell surface in the cortical regions of APP/PS1 mice injected with AAV‐SV2A or AAV‐Con. The protein levels of APP on the cell surface were measured by the cell surface biotin assay. Data are presented as the mean ± SD. All dot plots: t ‐test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The following primary antibodies were used in the study, early endosome antigen 1 (EEA1) (Cat. No. sc‐137,130, Santa Cruz, 1:100); APP (Cat. No. ab32136, Abcam, 1:2000; Cat. No. MAB348, Sigma‐Aldrich, 1:400); BACE1 (Cat. No. PA1‐757, Thermo Fisher Scientific, 1:200); Ras‐related protein Rab‐7 (Rab7) (ab137029, Abcam, 1:100); Ras‐related protein Rab‐11 (Rab11) (Cat. No. 5589, Cell Signaling Technology, 1:100); Lysosome‐associated membrane protein‐1 (LAMP1) (Cat. No. 99437, Cell Signaling Technology, 1:100).

    Techniques: Over Expression, Control, Expressing, Fluorescence, Transfection, Injection